Case Studies

 
Cytokines drive autoimmune illness and recovery

Hyperbaric Oxygen Therapy ImmunoTherapy Modulates Gene Expression

Oxygen In - Garbage OUT ('Wash-out')

Adv Drug Deliv Rev. 2017 Feb 15. pii: S0169-409X(17)30028-5. doi: 10.1016/j.addr.2017.02.001. [Epub ahead of print]

Mimicking Oxygen delivery and waste removal functions of blood.

Zhang H1, Barralet JE2.

Author information

Abstract

In addition to immunological and wound healing cell and platelet delivery, ion stasis and nutrient supply, blood delivers oxygen to cells and tissues and removes metabolic wastes. For decades researchers have been trying to develop approaches that mimic these two immediately vital functions of blood. Oxygen is crucial for the long-term survival of tissues and cells in vertebrates. Hypoxia (oxygen deficiency) and even at times anoxia (absence of oxygen) can occur during organ preservation, organ and cell transplantation, wound healing, in tumors and engineering of tissues. Different approaches have been developed to deliver oxygen to tissues and cells, including hyperbaric oxygen therapy (HBOT), normobaric hyperoxia therapy (NBOT), using biochemical reactions and electrolysis, employing liquids with high oxygen solubility, administering hemoglobin, myoglobin and red blood cells (RBCs), introducing oxygen-generating agents, using oxygen-carrying microparticles, persufflation, and peritoneal oxygenation. Metabolic waste accumulation is another issue in biological systems when blood flow is insufficient.

Metabolic wastes change the microenvironment of cells and tissues, influence the metabolic activities of cells, and ultimately cause cell death. This review examines advances in blood mimicking systems in the field of biomedical engineering in terms of oxygen delivery and metabolic waste removal.

OXYMED Case Study - Ms ML age 57 - Traumatic Brain Injury

  • SPECT (Single Photon Emission Tomography) demonstrate large perfusion defect inferior cortex of the right cerebellar lobe. Reduced perfusion of the pons and right thalamus.

  • Cytokine Profile - Base line pre-HBO (left) and after 50-hours HBO (right).

  • As HBOT penetrates hypoxic damaged cells there is a 'washout' effect of inflammatory cytokines IL8 including IL1, IL7, TNFa

  • As HBOT penetrates hypoxic damaged cells there is a positive gene expression ie elevation of anti inflammatory cytokines - HBO responsive elevation of the anti-inflammatory cytokines - GM-CSF, IL10, IL13, INFg.

  • It is expected with further HBO to observe a reduction of the inflammatory washout with continuing elevation of the anti-inflammatory cytokines expressive of reparative gene expression and neuroplasticity.

OXYMED Case Study - Mr GP age 64 - Right Middle Cerebral Artery Stroke

  • Cytokine Pre-HBO and Post-HBO - note significant elevation pre-HBO IL1, IL7, IL8 and TNFa.

OXYMED Case Study - Mr DH age 58 - Shoulder Reconstruction, Back Injury

  • Cytokine Pre-HBO and Post-HBO - note significant elevation post-HBO GM-CSF, IL4, IL10, IL13, INFg

OXYMED Case Study - Dr JM age 63 - Traumatic Brain Injury, Cranial Nerve Damage, Shoulder Rotator Cuff Tear

  • Cytokine Pre-HBO and Post-HBO - note 'washout' of IL8 and elevation GM-CSF, IL10, IL13.

OXYMED Case Study - Mr TC age 28 - Diffuse Traumatic Brain Injury

  • Cytokine Pre-HBO and Post-HBO - note 'washout' of IL1, IL7, IL8 and responsive elevation GM-CSF, IL4, IL10, IL13 and INFg.

OXYMED Case Study - Mr PK age 25 - Diffuse Traumatic Brain Injury

  • Cytokine Pre-HBO and Post-HBO - note 'washout' of IL8 and responsive elevation GM-CSF, IL10 and INFg.

OXYMED Case Study - World Class Athlete - Growing Injury List

  • Cytokine Pre-HBO note significant elevation of pro-inflammtory cytokines IL1, IL6, IL7, IL8 and TNFa.

  • This athlete is at the business end of his sporting career and confronted by chronic injuries. His blood profile is possibly worse that most disabled patients attending!

  • For more go to Sports Recovery & Performance

OXYMED Case Study - Mr DA - Glioblastoma

  • Cytokine Pre-HBO and Post-HBO at intervals. Patient was undertaking interval radiation in conjunction with HBOT.

  • For more go to GM-CSF and Cancer Support

OXYMED Case Study - Mr DB - Traumatic Brain Injury, Chronic Lyme Like Illness

  • Cytokine Pre-HBO and Post-HBO at intervals. Note significant response of GMCSF

  • For more go to GM-CSF 

OXYMED Case Study - Ms SR - Metastatic Cancer Multiple Organs

  • Cytokine Pre-HBO and Post-HBO at intervals. Note elevation response of GM-CSF.

  • For more go to GM-CSF 

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